Centre for Cellular Imaging

From CryoCapCell.

From CryoCapCell.

This fixed charge covers between 8 and 24 hours of imaging using the Array tomography (Atlas software) on our Gemini II 450 SEM. The charge includes staff assistance time. 

Pair of C-clip (1036171, ThermoFisher) and C-clip rings (1036173, ThermoFisher) used for clipping grids for the autoloader.

1036171, Thermo Scientific

Charged per run using our Quorum 150T SE.

Preparations in this category are designed to preserve specimen anitgenicity. They include only primary fixation (typically with formaldehyde), dehydration and embedding in acrylic resin such as LR White.  

CPD is performed with the K850 Critical Point Drier (Quorum Technology) to remove dehydration solvents from samples without causing surface morphology artifacts, before gold-coating for the SEM. 

Cryo-EM screening of ice thickness and quality as well as particle distribution. The charge covers a day of instrument (Talos L120C) and staff time at a discounted price. 

HPF + LN2 + staff time included. Consumables not included.

HPF + LN2 + staff time included. Consumables not included. 

From CryoCapCell. The exclusive tool specially developed for live imaging before freezing.

From Menzel-Glaser (01 1101)

From Epredia, cut (AA00000102E01MNZ10)

From Epredia, cut frosted (AA00000112E01MNZ10)

From Menzel-Glaser (J3800AMNZ, ThermoFisher)

GloCube Plus (Quorum). This procedure renders specimen grid film and other substrates such as silica hydrophilic and allows better specimen adhesion.

Circular gridbox from ThermoFisher. Compatible with Vitrobot mark IV.

Circular gridbox compatable vith vitrobot mark IV (AGG3736, Agar Scientific).

Gridbox for room temperature storage, 100 grid capacity.

Carbon Film 300 Mesh, Cu, 5-6 nm carbon (EMS product number: CF300-CU-50).

Quantifoil R 1.2/1.3 (Q3100CR1.3, EMS).

Quantifoil R 2/2 (Q3100CR2, EMS).

Gilder Grids, 100 mesh, 3.05mm O.D., Center and Rim-Mark, Copper (G100, Ted Pella)

Gilder Grids, 150 mesh, 3.05mm O.D., Center and Rim-Mark, Copper (G150, Ted Pella)

PELCO® Center-Marked Grids, 200 mesh, 3.0mm O.D., Copper (1GC 200, Ted Pella)

Gilder Hexagonal Grids, 100 mesh, 3.05mm O.D., Copper (G100HEX, Ted Pella)

Gilder Hexagonal Grids, 150 mesh, Rim-Mark, 3.05mm O.D., Copper (G150HEX, Ted Pella)

Gilder Hexagonal Grids, 200 mesh, 3.05mm O.D., Copper (G200HEX, Ted Pella)

PELCO® Center-Marked Grids, 150 mesh, 3.0mm O.D., Nickel (1GN150, Ted Pella)

PELCO® Center-Marked Grids, 200 mesh, 3.0mm O.D., Nickel (1GN200, Ted Pella)

Gilder Hexagonal Grids, 100 mesh, 3.05mm O.D., Nickel (G100HEX-N, Ted Pella)

PELCO^® Slot Grids, 1 x 2mm, 3.0mm O.D., Copper (1GC12H, Ted Pella)

PELCO^® Slot Grids, 1 x 2mm, 3.0mm O.D., Nickel (1GN12H, Ted Pella)

Formvar/Carbon Film, 200 Mesh Nickel, (AGS162N, Agar Scientific).

Each user must provide the primary antibody as well as demonstrate that it works for light microscopy lebeling (i.e. fluorescent microscopy). Using the same primary antibody we'll release 8-10 grids per run.

The immuno-EM technique (e.g. gold enhancement, HRP-DAB, double labelling) will be discussed individually with the user to choose the best approach. Each user must provide the primary antibody and demonstrate that it works at light microscopy level (i.e. fluorescent microscopy). We process up to 10 grids per run. 

Indium-tin-oxide coated glass slides from Technistro (Article number: TIX001)

Dimension: 25mm x 25mm x T1.1mm

Resistivity: ≤ 10 ohms/sq.

Tansmittence: ≥ 90%

HSN Code: 70200019

Brand: TECHNISTRO

Product code: TIX001  

https://www.techinstro.com/shop/conductive-glass/ito-coated-glass-slides/

FS + LN2 + consumables included. Staff time not included.

FS + LN2 + consumables included. Staff time not included.

Charge per run.

From MatTek (P35G-1.5-14-G-Grid).

From MatTek (P35G-1.5-14-C).

Stub: Standard Pin Stub SEM Mount with Slotted 1/2" Head (EMS product number: 75230)

Carbon tab: PELCO Tabs™, Carbon Conductive Tabs, 12mm OD (TadPella product number: 16084-1)

Stub: SEM Specimen Stubs, 25mm dia, 3.2 x 8mm pin (AgarScientific product number: AGG399)

Carbon tab: PELCO Tabs™, Carbon Conductive Tabs, 25mm OD (TadPella product number: 16084-2)

Stub: Standard Pin Stub SEM Mount Small Slotted Head 32 mm (EMS product number: 75189)

Carbon tab: PELCO Tabs™, Carbon Conductive Tabs, 32mm OD (TadPella product number: 16084-32)

Price per sample, does not include critical point drying or sputter-coating (charged separately). 

Price per sample, does not include critical point drying or sputter-coating (charged separately). Osmium- Thiocarbohydrazide-Osmium-Thiocarbohydrazide-Osmium (OTOTO) staining technique provides increased conductivity compared with standard single osmium and O-T-O treatment.  

PELCO® 18 SEM Pin Mount Storage Holder and Box (16709, Ted Pella).

Price per sample, does not include critical point drying or sputter-coating (charged separately). Osmium-Thiocarbohydrazide-Osmium (OTO) staining technique provides increased conductivity compared with standard single osmium treatment.  

From MicroToNano (10-008149).

From MicroToNano (10-008145).

From MicroToNano (10-008140).

MicroToNano (wafer) + Rowaco (C-tape).

Includes chemicals (Uranyl acetate, Uranyl formate, etc) charged per batch of up to 10 grids.

Charged per sample. Includes chemicals needed.

Price per sample, does not include critical point drying or sputter-coating (charged separately). Osmium- Thiocarbohydrazide-Osmium-Thiocarbohydrazide-Osmium (OTOTO) staining technique provides increased conductivity compared with standard single osmium and O-T-O treatment and samples are embedded in Epoxy or Acyrilic resins. 

Charged per sample. Includes chemicals needed.

Steel 110mm #5 Biology grade, SS, Polished. DUMONT Prod. n°. 505

Steel 108mm #5N Biology grade, SS. DUMONT Prod. n°. 515

Includes cryo-sectioning on a Leica UC6 with a Diatome diamond knife. Does not include consumables or staff time.

Includes sectioning on a Leica UC6/UC7 with a Diatome diamond knife. Does not include consumables (grids, wafers, etc) or staff time.

Custom made sample preparation training depending on user's sample.

1 hour training on how to perform negative stain sample preparation at CCI

Custom made sample preparation training depending on user's sample.

2-3 hour training on the Ultracut E (Reichert-Jung). 

A 3 hour introduction aimed at beginners to our TEM (Talos L120C, ThermoFisher) or SEM (GeminiSEM 450, Zeiss).

Follow-up training for more indepedent use of the systems.

Custom made user training for special modules of the TEM or SEM systems.

1 hour training. Vibratome sectioning allows for thin specomen slices to be obtained either for imaging directly or for further processing, to facilitate better penetration of reagents. 

3 hour training on how to operate the vitrobot mark IV (ThermoFisher).

A 3 hour introduction aimed at beginners to our TEM (Talos L120C, ThermoFisher) or SEM (GeminiSEM 450, Zeiss).

Follow-up training for more indepedent use of the systems.

Custom made user training for special modules of the TEM or SEM systems.

1 hour training on how to perform negative stain sample preparation at CCI

Custom made sample preparation training depending on user's sample.

Custom made sample preparation training depending on user's sample.

2-3 hour training on the Ultracut E (Reichert-Jung). 

1 hour training. Vibratome sectioning allows for thin specomen slices to be obtained either for imaging directly or for further processing, to facilitate better penetration of reagents. 

3 hour training on how to operate the vitrobot mark IV (ThermoFisher).

From CryoCapCell.

From CryoCapCell.

1036171, Thermo Scientific

Pair of C-clip (1036171, ThermoFisher) and C-clip rings (1036173, ThermoFisher) used for clipping grids for the autoloader.

From CryoCapCell. The exclusive tool specially developed for live imaging before freezing.

From Menzel-Glaser (01 1101)

From Epredia, cut (AA00000102E01MNZ10)

From Epredia, cut frosted (AA00000112E01MNZ10)

From Menzel-Glaser (J3800AMNZ, ThermoFisher)

Carbon Film 300 Mesh, Cu, 5-6 nm carbon (EMS product number: CF300-CU-50).

Gilder Grids, 100 mesh, 3.05mm O.D., Center and Rim-Mark, Copper (G100, Ted Pella)

Gilder Grids, 150 mesh, 3.05mm O.D., Center and Rim-Mark, Copper (G150, Ted Pella)

PELCO® Center-Marked Grids, 200 mesh, 3.0mm O.D., Copper (1GC 200, Ted Pella)

Gilder Hexagonal Grids, 100 mesh, 3.05mm O.D., Copper (G100HEX, Ted Pella)

Gilder Hexagonal Grids, 150 mesh, Rim-Mark, 3.05mm O.D., Copper (G150HEX, Ted Pella)

Gilder Hexagonal Grids, 200 mesh, 3.05mm O.D., Copper (G200HEX, Ted Pella)

PELCO® Center-Marked Grids, 150 mesh, 3.0mm O.D., Nickel (1GN150, Ted Pella)

PELCO® Center-Marked Grids, 200 mesh, 3.0mm O.D., Nickel (1GN200, Ted Pella)

Gilder Hexagonal Grids, 100 mesh, 3.05mm O.D., Nickel (G100HEX-N, Ted Pella)

PELCO^® Slot Grids, 1 x 2mm, 3.0mm O.D., Copper (1GC12H, Ted Pella)

PELCO^® Slot Grids, 1 x 2mm, 3.0mm O.D., Nickel (1GN12H, Ted Pella)

Formvar/Carbon Film, 200 Mesh Nickel, (AGS162N, Agar Scientific).

Quantifoil R 1.2/1.3 (Q3100CR1.3, EMS).

Quantifoil R 2/2 (Q3100CR2, EMS).

Circular gridbox compatable vith vitrobot mark IV (AGG3736, Agar Scientific).

Circular gridbox from ThermoFisher. Compatible with Vitrobot mark IV.

Gridbox for room temperature storage, 100 grid capacity.

Indium-tin-oxide coated glass slides from Technistro (Article number: TIX001)

Dimension: 25mm x 25mm x T1.1mm

Resistivity: ≤ 10 ohms/sq.

Tansmittence: ≥ 90%

HSN Code: 70200019

Brand: TECHNISTRO

Product code: TIX001  

https://www.techinstro.com/shop/conductive-glass/ito-coated-glass-slides/

From MatTek (P35G-1.5-14-C).

From MatTek (P35G-1.5-14-G-Grid).

From MicroToNano (10-008145).

From MicroToNano (10-008149).

From MicroToNano (10-008140).

MicroToNano (wafer) + Rowaco (C-tape).

PELCO® 18 SEM Pin Mount Storage Holder and Box (16709, Ted Pella).

Stub: Standard Pin Stub SEM Mount with Slotted 1/2" Head (EMS product number: 75230)

Carbon tab: PELCO Tabs™, Carbon Conductive Tabs, 12mm OD (TadPella product number: 16084-1)

Stub: SEM Specimen Stubs, 25mm dia, 3.2 x 8mm pin (AgarScientific product number: AGG399)

Carbon tab: PELCO Tabs™, Carbon Conductive Tabs, 25mm OD (TadPella product number: 16084-2)

Stub: Standard Pin Stub SEM Mount Small Slotted Head 32 mm (EMS product number: 75189)

Carbon tab: PELCO Tabs™, Carbon Conductive Tabs, 32mm OD (TadPella product number: 16084-32)

Steel 110mm #5 Biology grade, SS, Polished. DUMONT Prod. n°. 505

Steel 108mm #5N Biology grade, SS. DUMONT Prod. n°. 515

Cryo-EM screening of ice thickness and quality as well as particle distribution. The charge covers a day of instrument (Talos L120C) and staff time at a discounted price. 

This fixed charge covers between 8 and 24 hours of imaging using the Array tomography (Atlas software) on our Gemini II 450 SEM. The charge includes staff assistance time. 

Charged per run using our Quorum 150T SE.

Preparations in this category are designed to preserve specimen anitgenicity. They include only primary fixation (typically with formaldehyde), dehydration and embedding in acrylic resin such as LR White.  

CPD is performed with the K850 Critical Point Drier (Quorum Technology) to remove dehydration solvents from samples without causing surface morphology artifacts, before gold-coating for the SEM. 

GloCube Plus (Quorum). This procedure renders specimen grid film and other substrates such as silica hydrophilic and allows better specimen adhesion.

Each user must provide the primary antibody as well as demonstrate that it works for light microscopy lebeling (i.e. fluorescent microscopy). Using the same primary antibody we'll release 8-10 grids per run.

The immuno-EM technique (e.g. gold enhancement, HRP-DAB, double labelling) will be discussed individually with the user to choose the best approach. Each user must provide the primary antibody and demonstrate that it works at light microscopy level (i.e. fluorescent microscopy). We process up to 10 grids per run. 

Price per sample, does not include critical point drying or sputter-coating (charged separately). 

Price per sample, does not include critical point drying or sputter-coating (charged separately). Osmium-Thiocarbohydrazide-Osmium (OTO) staining technique provides increased conductivity compared with standard single osmium treatment.  

Price per sample, does not include critical point drying or sputter-coating (charged separately). Osmium- Thiocarbohydrazide-Osmium-Thiocarbohydrazide-Osmium (OTOTO) staining technique provides increased conductivity compared with standard single osmium and O-T-O treatment.  

Includes chemicals (Uranyl acetate, Uranyl formate, etc) charged per batch of up to 10 grids.

Charged per sample. Includes chemicals needed.

Charged per sample. Includes chemicals needed.

Price per sample, does not include critical point drying or sputter-coating (charged separately). Osmium- Thiocarbohydrazide-Osmium-Thiocarbohydrazide-Osmium (OTOTO) staining technique provides increased conductivity compared with standard single osmium and O-T-O treatment and samples are embedded in Epoxy or Acyrilic resins. 

Includes cryo-sectioning on a Leica UC6 with a Diatome diamond knife. Does not include consumables or staff time.

Includes sectioning on a Leica UC6/UC7 with a Diatome diamond knife. Does not include consumables (grids, wafers, etc) or staff time.

HPF + LN2 + staff time included. Consumables not included.

HPF + LN2 + staff time included. Consumables not included. 

FS + LN2 + consumables included. Staff time not included.

FS + LN2 + consumables included. Staff time not included.

Charge per run.